The Relative Response Factor (RRF) is an analytical measure used in chromatographic processes to monitor impurities/degradants in drug substances and drug products. RRF is utilized to compensate for the disparity in detector response of contaminants vs. analyte peak. It is established using the slope approach with a linear range of solutions.
Process-related impurities are undesirable substances found in biopharmaceutical samples. These chemicals can be cell substrates, come from the production process (e.g., nutritional medium, initiators of protein production and selection, chemicals utilized in further purification), or be the result of chemical alterations to the product.
Nuclear magnetic resonance spectroscopy (NMR) is a common and successful technique that uses the magnetic properties of individual nuclei. When exposed to an external magnetic field, some nuclei exhibit distinct nuclear spin states, which is the underlying premise of NMR. NMR identifies transitions between spin states specific to the nuclei in issue, as well as their chemical surroundings. However, this only applies to nuclei with spins other than zero; nuclei with spins equal to zero are 'invisible' to NMR spectroscopy.
One analytical method for determining an ion's mass-to-charge ratio is mass spectrometry (MS). A mass spectrum, or a plot of intensity vs mass-to-charge ratio, is used to display the results. Mass spectrometry is applicable to both complicated mixtures and pristine samples in numerous fields.
Drug storage is one of the pharmacist's most crucial responsibilities. As a result, suitable means for ensuring compliance with these responsibilities must be created and executed.
It is a phenomenon in which two or more compounds share the same chemical formula but have different structural formula and properties. This is primarily due to variations in structural or spatial arrangements.
Types of Isomerism:
Structural Isomerism
Stereo Isomerism
Infrared spectroscopy is the study of how infrared light interacts with molecules. This can be assessed in three different ways: absorption, emission, and reflection. This technique is most commonly employed in organic and inorganic chemistry. Chemists use it to identify functional groups in molecules. IR Spectroscopy measures the vibrations of atoms, which The IR transmitter continuously emits IR light, while the IR receiver constantly detects reflected light.
Inert gases are chemically inactive and will not react with many things. Inert gases are utilized for a variety of applications throughout sectors, including welding, chemical processing, and filler gases in light sources. They are: helium, neon, argon, krypton, xenon, and radon.
High performance liquid chromatography, also known as high-pressure liquid chromatography, is a chromatographic technique for separating a mixture of substances that is used in biochemistry and analytical chemistry to identify, measure, and purify the mixture's constituent components. A liquid sample is injected into a solvent stream (mobile phase) that flows through a column containing a separation medium (stationary phase). As samples travel through the column, they separate from one another through a process known as differential migration.
An analytical method that can be used on gas, liquid, and solid samples (components that evaporate due to heat) is gas chromatography (GC). When a GC system is used to investigate a mixture of compounds, each compound can be identified and measured separately.